ʻO ka Ethylenediaminetetraacetic acid (EDTA) kahi mea hana chelating metala. ʻO ka mea i kapa ʻia ʻo chelating, ʻo ia hoʻi, hiki iā ia ke hui pū me kekahi mau ion metala a hana ʻole i nā ion metala i hoʻopaʻa ʻia.

Ke hoʻohana nei i kēia ʻano o EDTA, ʻelua ʻano o nā noi i nā hoʻokolohua;

Hoʻokahi ʻano ʻo ia ka hoʻohana ʻana i ka huli ʻana, ka mea e pale ai i kekahi mau hopena ma o ka hoʻopili ʻana i ka hoʻopaʻa ʻana o nā ion metala.

E lawe i kahi ion: I kekahi mau hopena, he mea nui ka hoʻopaʻa ʻana o nā ion metala. Hoʻohui mākou i ka EDTA me kēia mau ion metala e pale i kēia pane.

No ka laʻana, pono ka coagulation koko i ke komo ʻana o nā ion calcium. Hoʻohui mākou i ka EDTA i ke koko e chelate i nā ion calcium me EDTA, pēlā e pale ai i ka coagulation koko. ʻO kēia ke kumu o ka EDTA anticoagulation;

No kekahi laʻana, hoʻohana pū ka hoʻomākaukau ʻana i ka hopena decalcification i ka staining pathological i ka hopena chelation o EDTA. Ua ʻike mākou inā ʻaʻole i hoʻokaʻawale ʻia ka ʻiʻo calcium kiʻekiʻe, e paʻakikī ke ʻoki ʻia i loko o ka ʻiʻo piha i ka wā e ʻoki ai, no laila pono mākou e decalcify ma mua o ka ʻoki ʻana.

Aia kekahi ʻano o ka noi mua e hoʻoikaika i kekahi mau hopena ma ke keʻakeʻa ʻana i ka paʻa ʻana o nā ion metala.

No ka laʻana, hoʻohui i ka EDTA i ka hoʻonā trypsin digestion. Inā ʻaʻole i hoʻohui ʻia ʻo EDTA, e hoʻopaʻa ʻia ka trypsin i ka calcium a me ka magnesium ma ka ʻili o ke kelepona a i ʻole ka moʻomeheu moʻomeheu, a laila e pale ai i ka hana trypsin.

I kēia manawa, e hoʻohui i kahi wahi liʻiliʻi o EDTA e ʻae iā EDTA e hui pū me kēlā mau calcium manuahi, magnesium a me nā ion divalent ʻē aʻe. I kēia manawa, hana ʻia kahi kūlana maikaʻi no ka hiki ʻana o trypsin.

ʻO ka mea kupanaha loa e pili ana i ka EDTA ʻo ia kona solubility. Hiki ke hoʻoheheʻe ʻia i ka wai a ʻike ʻia ke keʻokeʻo i ka wai. I kēia manawa, e hoʻohui i ka sodium hydroxide e hoʻoponopono i kona waiwai pH. I ka piʻi ʻana o ka pH a ʻoi aku ka kiʻekiʻe, lilo ka hopena E lilo i mea akaka a maʻalahi. Ke kokoke ka pH i 8, akaka loa. ʻAʻole makaʻu i ka waika akā alkali.

Nui nā hoʻohana nani ʻo EDTA. Hiki ke ʻike ʻia he kupua loa nā mea ma ke ʻano, akā ʻaʻole mākou i ʻike.

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